Development of a high-throughput infection bioassay
Together with Dr. Chunqui Zhang a visiting scholar in our lab I have been developing a way to quantitatively screen for disease resistance in a high-throughput manner. Tissue plugs from cucumber fruit are placed in a 96-well plate and inoculated with a constitutively expressed fluorescent tag. The plates are then read in a Teccan Plate reader and the fluorescent signal is used as a proxy for pathogen growth or - in the case of resistant lines - lack thereof. The method allows for real-time tracking of disease symptoms in live tissue and in high replication. Application of this method could be in screening large populations or treatments.